The Indian Council for Medical Research (ICMR) has approved a simple and fast method for Covid-19 testing which not only increase the number of RT-PCR tests but can also bring down costs. The method is known as Dry Swab-Direct RT-PCR and is developed by CSIR’s Centre for Cellular and Molecular Biology in Hyderabad.
The Indian Council for Medical Research (ICMR) has approved a simple and fast method for Covid-19 testing, the Council for Scientific and Industrial Research (CSIR) said on Saturday. This method can not only increase the number of RT-PCR tests but can also bring down costs, it said.
The method — Dry Swab-Direct RT-PCR — developed by CSIR’s Centre for Cellular and Molecular Biology (CCMB) in Hyderabad, is a variation of the existing gold standard RT-PCR method and can easily scale up the testing by 2 to 3 fold with no new investment of resources.
“After evaluating this method and finding an overall concordance of 96.9 per cent, the ICMR has now issued an advisory for the use of CSIR-CCMB dry swab method, considering its lesser cost and quick turn-around time,” PTI quoted the CSIR as saying.
According to PTI, the CCMB has been testing samples for coronavirus since April. Having worked closely with healthcare workers of Telangana, it identified some of the key issues that slow the testing process. In response to it, the researchers developed the Dry Swab RNA-extraction free testing method for Covid-19.
HOW DRY SWAB-DIRECT RT-PCR METHOD WORKS
In the Dry Swab-Direct RT-PCR method, the nasal swab is collected and transported in dry state, as opposed to using the viral transport medium VTM, which makes the transportation and handling of the samples easy and less prone to spillage and spread of infection.
Secondly, the step of RNA isolation from the sample is omitted and involves only simple processing of the sample followed by direct RT-PCR using the kit recommended by the ICMR.
“Omitting the step of RNA isolation offers a huge benefit over the conventional method, as the RNA isolation is a major bottleneck in terms of time, cost and trained manpower. Given this, with the same resources and no additional cost, more samples can be tested and can be easily scaled up at least 2-3 times immediately,” the CSIR was quoted as saying.
Rakesh Mishra, director, CCMB said that RNA extraction, even with automation, takes four hours for roughly 500 samples.
“VTM and RNA extraction both add a significant burden on money and time required for mass testing for coronavirus. We believe the technique’s merit holds for all kinds of settings and has the potential of bringing the costs and time of testing by 40-50 per cent,” the CSIR said.
CSIR director general Shekhar Mande said that the Dry-Swab Direct RT-PCR method is cost effective, easy to implement with no requirement of new kits and existing manpower can perform this with no additional training and hence could make a significant contribution to ramping up the testing capacity in the country quickly.
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